金蛤口服液对环磷酰胺所致血虚模型小鼠外周血象及记忆功能的影响

Influences of Jinha Oral Liquid on Peripheral Blood and Memory Function in Model Mice of Hematopenia Induced by Cyclophosphamide

目的:研究金蛤口服液对血虚模型小鼠外周血象及记忆功能的影响。方法:给予小鼠腹腔注射环磷酰胺50mg/kg和照射X射线复制血虚模型。实验分为空白对照(等容生理盐水)、模型(等容生理盐水)、红参黄芪(10g/kg)与金蛤口服液高、中、低剂量(53、26.5、13.3g/kg)组,灌胃给药,每天1次,连续21d。给药第14天和第21天测定小鼠外周血象。血虚模型小鼠腹腔注射氢溴酸东莨菪碱4mg/kg致记忆受损,记录小鼠跳台实验中潜伏期、错误次数。结果:给药第14天,与空白对照组比较,模型组白细胞(WBC)、红细胞(RBC)显著减少(P<0.05);与模型组比较,金蛤口服液高、中、低剂量组小鼠WBC显著增加,金蛤口服液高、中剂量组RBC显著增加(P<0.05)。给药第21天,与空白对照组比较,模型组WBC、RBC、血红蛋白(HGB)显著减少(P<0.05);与模型组比较,金蛤口服液高、中、低剂量组小鼠WBC显著增加,金蛤口服液高剂量组RBC显著增加,金蛤口服液高、低剂量组HGB显著增加(P<0.05)。与空白对照组比较,模型组潜伏期显著缩短,错误次数显著增加(P<0.05);与模型组比较,金蛤口服液高、中、低剂量组潜伏期显著延长,错误次数显著减少(P<0.05)。结论:金蛤口服液对血虚模型小鼠外周血血细胞状况尤其是白细胞有明显的改善作用,对小鼠记忆功能可能有改善作用。

OBJECTIVE： To study the influences of Jinha oral liquid on peripheral blood and memory function in model mice of hematopenia induced by cyclophosphamide. METHODS： Deficiency of blood mice model was induced by cyclophosphamide 50 mg/kg intraperitoneally and irradiated with X-rayscyclophosphamide. Mice were divided into blank control group （constant volume of normal saline）, model group （constant volume of normal saline）, Panax ginseng Astragali Radix group （10 g/kg） and Jinha oral liquid high-dose, medium dose and low-dose groups （53, 26.5, 13.3 g/kg）. They were given medicine intragastrically once a day for consecutive 21 days. Peripheral hemogram of mice was determined on 14 th and 21th day. Memory impairment blood model mice was given scopolamine hydrobromide 4 mg/kg intraperitoneally, latent period and error number of step-down test were record- ed. RESULTS： Compared with blank control group, white blood cells and red blood cells counts in model group were significantly lowered （P〈0.05） on 14th. Compared with model group, white blood cells in Jinha oral liquid high-dose, medium dose and low-dose groups were significantly highered , and red blood cells counts Jinha oral liquid high-dose, medium dose groups were significantly highered （P〈0.05） on 14th. Compared with blank control group, white blood cells, red blood cells and hemoglobin counts in model group were significantly lowered（P〈0.05） on 21th. Compared with model group, white blood cells counts in Jinha oral liquid high-dose, medium dose and low-dose groups were highered significantly highered, red blood cells counts Jinha oral liquid high-dose dose group were highered significantly highered, and hemoglobin counts in Jinha oral liquid high-dose and low-dose groups were highered significantly（P〈0.05） on 21th. Compared with blank control group, latent period in model group were shortened and error number of step-down test were increased significantly（P〈0.05）. Compared with model group, latent period were longhened in Jinha oral liquid high-dose, medium dose and low-dose groups, and error number of step-down test were decreased significantly （P〈0.05）. CONCLUSION： Jinha oral liquid can improve peripheral blood of mice model of hematopenia induced by cyclophosphamide, especially white blood cells, and also improve the memory function.