改良RP-HPLC法同时测定牡丹皮药材中芍药苷和丹皮酚的含量

Simultaneous Determination of Paeoniflorin and Paeonol in Paeonia suffruticosa by Modified RP-HPLC

目的:通过改良同时测定牡丹皮药材中芍药苷和丹皮酚含量的方法,为控制牡丹皮药材的质量提供更有效的手段。方法:色谱柱为Agilent ZORBAX Eclipse XDB C18(250mm×4.6mm,5μm),流动相为甲醇-水(梯度洗脱),流速为1.0ml/min,双波长检测(芍药苷为230nm,丹皮酚为274nm)。结果:芍药苷、丹皮酚的进样量分别在0.06033～1.2066、0.2004～4.0080μg范围内与各自峰面积积分值呈良好线性关系(r均为0.9999);精密度、重复性、稳定性试验的RSD均<2%;平均加样回收率分别为99.49%、98.66%,RSD分别为2.0%、1.8%(n均为6)。结论:改良方法去除了磷酸对色谱柱的腐蚀,方法简便、快速,结果准确,可为牡丹皮及其相关制剂中芍药苷和丹皮酚的定量分析提供参考。

OBJECTIVE： To provide more effective means for the quality control of Paeonia suffruticosa by improving the method for simultaneous determination of paeoniflorin and paeonol in P suffruticosa. METHODS ： Agilent ZORBAX Eclipse XDB C18 （250mm×4.6mm,5μm） column was used with mobile phase consisted of methanol-water （gradient elution） at the flow rate of 1.0 ml/min. The detection wavelength was 230 nm for paeoniflorin and 274 nm for paeonol. RESULTS ： The linear range of paeoniflorin was 0.060 33-1.206 6 μg and that of paeonol was 0.200 4-4.008 0 μg（r=0.999 9）. RSDs of precision test, reproducibility test and stability test were all lower than 2%. The average recoveries were 99.49% and 98.66% with RSDs of 2.0% and 1.8% respectively（n=6）. CONCLUSION： The modified method removes phosphate corrosion for chromatographic column. The method is simple, rapid and accurate, and can be used for the qualitative analysis of paeoniflorin and paeonol in P suffruticosa and its preparations.