摘要
Bt是转基因抗虫玉米育种应用的主要外源基因,包含编码杀虫晶体蛋白(ICPs)的Cry类和Cyt类基因,以及编码营养期杀虫蛋白(Vip)的vip类基因。迄今已有40余种转Bt基因玉米事件被26个国家批准进入商业化种植或饲料食品加工。目前,Bt基因研发仍在进行,并逐步向分离克隆和改造新基因以及构建高效表达载体方向发展。转Bt基因育种工程逐渐扩大靶标昆虫抗性范围,并向复合性状抗虫玉米发展。转化技术亦向高效化和安全化发展。综述了Bt基因作用机理、转Bt基因玉米研发及应用,拟为我国转Bt基因抗虫玉米研发提供参考。
Bacillus thuringiensis （Bt） is the important exogenous genes for transgenic corn breeding for insect re- sistance, which contains code insecticidal crystal protein （ICPs） Cry and Cyt gene, as well as code vegetative phase insecticidal protein gene （Vip）. So far, more than 40 transgenic Bt corn events have been certificated for commer- cialization or used for food and siliage processing in 26 gress, and is mainly focusing on cloning and modifying countries. At present, the research of Bt genes is still in pro- new Bt genes as well as constructing efficient expression vec- tot. By using the technologies of efficient and safe gene transformation, the target range of insect resistance will be expanded and the stacked trait insect-resistant corn will be developed in Bt transgenic breeding study, the mechanism of Bt gene research and application of GM （ genetically modified） corn with Bt marized,which will provide useful information for the research of Bt corn in China. program. In this gene were sum-
出处
《作物杂志》
CAS
CSCD
北大核心
2013年第2期7-12,共6页
Crops
基金
转基因专项课题(2011ZX08011-003)
