期刊文献+

猪卵巢颗粒细胞中SIRT1基因表达量对卵泡闭锁的影响

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摘要 SIRT1是一种二氢尿嘧啶脱氢酶(NAD)依赖的具有去乙酰化酶活性的多功能转录调节因子。本研究分析不同闭锁阶段卵泡颗粒细胞中SIRT1基因表达规律性,探讨SIRT1基因表达量与卵泡发育的关系。采用免疫组织化学方法分析SIRT1蛋白表达定位,qRT-PCR方法分析SIRT1 mRNA表达量。结果表明,SIRT1蛋白在晚期闭锁卵泡中最高,健康卵泡中最低,SIRT1 mRNA表达规律性与SIRT1蛋白表达规律性一致。综合分析,颗粒细胞中SIRT1基因随着卵泡的闭锁表达量逐渐升高,SIRT1表达与猪卵巢卵泡发育存在一定相关性。
出处 《江苏农业科学》 北大核心 2013年第3期168-170,共3页 Jiangsu Agricultural Sciences
基金 江苏省自然科学基金(编号:BK2010466) 国家生猪现代产业技术体系建设专项(编号:CARS-36) 江苏省农业种质资源库项目
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参考文献10

  • 1Howitz K T, Bitterman K J, Cohen H Y, et al. Small molecule activa- tors of sirtuins extend Saccharomyces cerevisiae lifespan [ J ]. Nature, 2003,425 (6954) :191 - 196.
  • 2Ho C, van der Veer E, Akawi O, et al. SIRT1 markedly extends repli- cative lifespan if the NAD + salvage pathway is enhanced[J]. FEBS Lett,2009,583 ( 18 ) :3081 - 3085.
  • 3Vaziri H, Dessain S K, Ng Eaton E, et al. hSIR2 ( SIRT1 ) functions as an NAD - dependent p53 deacetylase [ J]. Cell, 2001,107 ( 2 ) : 149 -159.
  • 4Johnson A L, Langer J S, Bridgham J T. Sirtuins as a cell cycle - related and antiapoptotic protein in granulosa cells [ J ]. Endocrinology, 2004,143:3405 - 3413.
  • 5Kong X X,Wang R, Liu X J, et al. Function of SIRT1 in physiology [ J ]. Biochemistry (Most) ,2009,74 (7) :703 - 708.
  • 6Yao Y, Li H, Gu Y, et al. Inhibition of SIRT1 deacetylase suppresses estrogen receptor signaling[ J]. Carcinogenesis ,2010,31:382 - 387.
  • 7Elangovan S, Ramachandran S, Venkatesan N, et al. SIRT1 is essen- tial for oncogenic signaling by estregen/estrogen receptor alpha in breast cancer[ J]. Cancer Res,2011,71:6654 - 6664.
  • 8Lee J H, Song M Y, Song E K, et al. Overexpression of SIRT1 protects pancreatic beta - cells against cytokine toxicity by suppressing the nuclear factor - kappa signaling pathway [ J ]. Diabetes, 2009,58 : 344 -351.
  • 9Kolthur- Seetharam U, Teerds K, De Rooij D G, et al. The histone deacetylase SIRT1 controls male fertility in miee through regulation of hypothalamie- pituitary gonadotrepin signaling [ J ]. Biol Reprod, 2009,80,384 - 391.
  • 10Pang W J,Yang Y J,Yang G S,et al. Modtflation of Sirtl by resvera- trol and nieotinamide alters proliferation and differentiation of pig preadipocytes [ J ]. Mol Cell Bioehem,2008,307 (1/2) : 129 - 140.

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