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猪圆环病毒2型ORF2基因重组腺病毒质粒的快速构建

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摘要 人5型腺病毒载体属于复制缺陷型载体,由于该载体有许多优点,目前已经成为应用最广泛的载体之一。腺病毒载体的穿梭质粒和骨架质粒有同源序列,二者在重组酶的作用下进行同源重组构成重组腺病毒质粒。构建重组腺病毒的方法有许多种,最早是加拿大F.L.Gramham等建立的在真核细胞内通过骨架质粒和穿梭质粒的同源重组构建出腺病毒。
出处 《黑龙江畜牧兽医》 CAS 北大核心 2013年第4期101-103,共3页 Heilongjiang Animal Science And veterinary Medicine
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参考文献10

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