2007-2011年麻醉恢复室空气监测结果分析

Analysis of results of air monitoring in post anesthesia care unit between 2007 and 2011

目的回顾性分析2007-2011年麻醉恢复室的空气检测结果,探讨麻醉恢复室空气质量对麻醉恢复期患者发生感染等并发症的影响。方法依据麻醉恢复室的规章制度进行空气消毒,室内温度保持在22～25℃,相对湿度在40%～60%,将采集好空气样本的平板置于培养箱中进行培养,细菌鉴定采用法国生物梅里埃公司的VITEK微生物鉴定仪。结果共计应查样本数300份,实查278份,占92.67%;其中无菌样本110份,菌落数<200CFU/m3有134份,200～500CFU/m3有24份,>500CFU/m3有10份,平均菌落数(86.2±33.8)CFU/m3;共分离出175株病原菌,分布最多的3种为金黄色葡萄球菌、表皮葡萄球菌和铜绿假单胞菌,分别占30.29%、20.57%和16.57%。结论麻醉恢复室2007-2011年空气质量相对平稳,但仍存在微生物污染严重,应完善麻醉恢复室的消毒隔离制度,以降低感染发生率。

OBJECTIVE To retrospectively analyze the results of the air testing in the post anesthesia care unit （PACU） from 2007 to 2011 so as to explore the impact of the air quality on the incidence of such complication as the infections during the recovery from anesthesia. METHODS The air in the PACU was disinfected in accordance with the rules and regulations, the temperature in the room maintained between 22 ℃ and 25 ℃, with the relative humidity varying from 40% to 60%. The air samples on the tablet were cultured in the incubator, and the bacteri- al identification was performed by French BioMerieux VITEK microbial identification system. RESULTS A total of 300 samples were expected to be investigated, and 278 samples were actually investigated, accounting for 92.67%, among which there were 110 sterile samples, 134 samples with the bacterial colony count below 200 CFU/m3 , 24 samples with the bacterial colony counts ranging from 200 CFU/ms to 500 CFU/m3 , and 10 samples with the bacterial colony count above 500 CFU/m3 , with the mean bacterial colony count of （86.2±33.8） CFU/ m3. A total of 175 strains of pathogens were isolated, and the Staphylococcus aureus, Staphylococcus epidermidis and Pseudomonas aeruginosa were the three species of predominant pathogens, accounting for 30.29 %, 20.57 %, and 16.57%, respectively. CONCLUSION The air quality is relatively stable in the PACU during 2007--2011, however, the microbial contamination remains serious, thus the disinfection and isolation system should be made sound so as to reduce the incidence of infections.