黄芩茎叶中黄酮提取物对过氧化氢诱导的大鼠皮层神经元损伤的保护作用

Flavonoid extract from stem and leaf of Scutellaria baicalensis Georgi attenuates H_2O_2-induced oxidative damages in rat cortical neurons

目的:考察黄芩茎叶黄酮提取物(SSF)对过氧化氢(H2O2)诱导的大鼠原代皮层细胞损害的保护作用。方法:100μmol.L-1H2O2作用大鼠原代皮层细胞150 min,测定细胞存活率、形态、乳酸脱氢酶(lac-tate dehydrogenase,LDH)释放量、丙二醛(malondialdehyde,MDA)的产生、超氧化物歧化酶(superoxide dis-mutase,SOD)、谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-Px)/Na+-K+-ATP酶的活性以及SSF对H2O2引起大鼠原代皮层细胞上述指标异常改变作用。结果:H2O2明显降低大鼠原代皮层细胞存活率,使细胞形态发生异常变化,增加LDH和MDA生成,显著降低SOD/GSH-Px/Na+-K+-ATP酶的活性。18～76μg.mL-1SSF预处理细胞24 h明显反转上述变化。结论:SSF对H2O2引起的神经损伤具有保护作用,该作用有利于神经退行性疾病的治疗。

Objective： To investigate the effect of flavonoid extract from stem and leaf of Scutellaria ba- icalensis Georgi on H202-induced damage in primary rat cortical neurons. Methods： Rat cortical neurons were treated with 100 μmol·L-1 H202 for 150 min. Cell damage was evaluated by determining viability, morphology, lactate dehydrogenase （LDH） release, malondialdehyde （MDA） production, and the activities of superoxide dis- mutase （SOD）, glutathione peroxidase （GSH-Px） and Na＋-K＋-ATPase. Effects of the flavonoids extract on H2O2-indueed damage in the neurons were assessed. Results： After exposure of the neurons to H2O2 , cell viability reduced, abnormal changes in neuron morphology were found, LDH release and MDA production increased, and SOD, GSH-Px and Na＋-K＋-ATPase activities were decreased. Flavonoid extract （18-76μg·mL-1 for 24 h） at- tenuated H2 O2 -induced damage, as the cell survival, and SOD, GSH-Px and Na＋-K＋-ATPase activities were raised, and MDA production and LDH release were lowered. Conclusion： Flavonoid extract from stem and leaf of Scutellaria baicalensis Georgi has a protective effect on H2O2-induced neuronal damage, further supporting its ther- apeutic potentials for neurodegenerative diseases.