摘要
目的:考察黄芩茎叶黄酮提取物(SSF)对过氧化氢(H2O2)诱导的大鼠原代皮层细胞损害的保护作用。方法:100μmol.L-1H2O2作用大鼠原代皮层细胞150 min,测定细胞存活率、形态、乳酸脱氢酶(lac-tate dehydrogenase,LDH)释放量、丙二醛(malondialdehyde,MDA)的产生、超氧化物歧化酶(superoxide dis-mutase,SOD)、谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-Px)/Na+-K+-ATP酶的活性以及SSF对H2O2引起大鼠原代皮层细胞上述指标异常改变作用。结果:H2O2明显降低大鼠原代皮层细胞存活率,使细胞形态发生异常变化,增加LDH和MDA生成,显著降低SOD/GSH-Px/Na+-K+-ATP酶的活性。18~76μg.mL-1SSF预处理细胞24 h明显反转上述变化。结论:SSF对H2O2引起的神经损伤具有保护作用,该作用有利于神经退行性疾病的治疗。
Objective: To investigate the effect of flavonoid extract from stem and leaf of Scutellaria ba- icalensis Georgi on H202-induced damage in primary rat cortical neurons. Methods: Rat cortical neurons were treated with 100 μmol·L-1 H202 for 150 min. Cell damage was evaluated by determining viability, morphology, lactate dehydrogenase (LDH) release, malondialdehyde (MDA) production, and the activities of superoxide dis- mutase (SOD), glutathione peroxidase (GSH-Px) and Na+-K+-ATPase. Effects of the flavonoids extract on H2O2-indueed damage in the neurons were assessed. Results: After exposure of the neurons to H2O2 , cell viability reduced, abnormal changes in neuron morphology were found, LDH release and MDA production increased, and SOD, GSH-Px and Na+-K+-ATPase activities were decreased. Flavonoid extract (18-76μg·mL-1 for 24 h) at- tenuated H2 O2 -induced damage, as the cell survival, and SOD, GSH-Px and Na+-K+-ATPase activities were raised, and MDA production and LDH release were lowered. Conclusion: Flavonoid extract from stem and leaf of Scutellaria baicalensis Georgi has a protective effect on H2O2-induced neuronal damage, further supporting its ther- apeutic potentials for neurodegenerative diseases.
出处
《中国新药杂志》
CAS
CSCD
北大核心
2013年第7期837-841,共5页
Chinese Journal of New Drugs
基金
国家中医药管理局资助项目(02-03-ZP18)
首届河北省高校百名优秀创新人才支持计划资助项目