HPLC法测定替硝唑螺内酯乳膏的含量

Content determination of Tinidazole and Spironolactone Cream by HPLC

目的研究替硝唑螺内酯乳膏含量测定方法。方法采用高效液相色谱法同时测定替硝唑螺内酯乳膏中替硝唑和螺内酯的含量。结果替硝唑和螺内酯线性范围分别为40.11～481.32μg.mL-1(r=0.999 7)和6.07～72.84μg.mL-1(r=0.999 9);平均回收率分别为100.7%(RSD=0.70%,n=9)和101.3%(RSD=1.00%,n=9)。结论该方法能有效地控制替硝唑螺内酯乳膏的质量。

Objective To study the content determination methods for Tinidazole and Spironolactone Cream.Methods The contents of tinidazole and spironolactone in Tinidazole and Spironolactone Cream were determined simultaneously by HPLC.Results The linear ranges for tinidazole and spironolactone were 40.11-481.32 μg·mL-1（r=0.999 7） and 6.07-72.84 μg·mL-1（r=0.999 9） with the average recovery rates 100.7%（RSD=0.70%,n=9） and 101.3%（RSD=1.00%,n=9）,respectively.Conclusion The established method is reliable for quality control of Tinidazole and Spironolactone Cream.