复凝聚法制备4种壳聚糖微囊的比较

Comparison of the preparation methods of four chitosan microcapsules by complex coacervation

目的研究复凝聚法制备壳聚糖微囊并比较其性能。方法壳聚糖为基本囊材,与羧甲基纤维素钠、阿拉伯胶、明胶、海藻酸钠复凝聚制备微囊。以包封率、载药量、回收率为优化指标,通过单因素实验确定最佳囊材比,并考察不同微囊体外释药性能。结果 4种壳聚糖微囊平均包封率为30%～62%,粒径1～2μm,具有较好的体外缓慢释放性能。制备的壳聚糖-羧甲基纤维素钠最佳囊材比为1∶0.2,壳聚糖-阿拉伯胶、壳聚糖-明胶、壳聚糖-海藻酸钠微囊的囊材比均为1∶1。结论 4种壳聚糖复合微囊可采用相似制备工艺,其中综合性能指标较好的是壳聚糖-明胶微囊。

Objective To study the preparation of chitosan microspheres by complex coacervation method,and to compare their performance.Methods Microcapsules were prepared by chitosan with carmellose sodium,arabic gum,gelatin or sodium alginate.The single factor experimental design using the standard of drug-encapsulation,and drug-load,and recovery percent,was applied to optimize the ratio of wall materials.The performance of several microcapsule drug release in vitro was investigated.Results The average drug-encapsulation of the four kinds chitosan microcapsules was 30%-62% and size around 1-2 μm.The vitro release of microcapsules indicated that these microcapsules had good sustained release performance in vitro.The optimal ratio of wall materials was as follows：chitosan-carmellose sodium 1∶0.2,while the chitosan-arabic gum,the chitosan-gelatin or the chitosan-sodium alginate was 1∶1.Conclusion The microcapsules of composite chitosan could be prepared by using the similar process.The microcapsules of chitosan-gelatin better than other microcapsules.