广东地区幽门螺杆菌vacA基因亚型及其与胃肠疾病的关系

vacA Gene Subtypes of Helicobacter pylori in Guangdong Area and their Relationships with Gastrointestinal Diseases

探讨广东地区幽门螺杆菌（H．pylori）vacA基因亚型的流行情况及不同vacA亚型与H．pylori相关性胃肠疾病的关系。方法：自广东地区不同胃十二指肠疾病患者胃粘膜中分离得到191株H．pylori菌株，抽提各菌株总DNA，用特定引物对各菌株vacA基因的信号序列（s）及中间区等位基因（m）行聚合酶链反应（PCR）检测。结果：广东地区患者H．pylori的vaCA亚型有sla／m2、sla／mlb、slb／m2、sla／mlb-m2、slb／mlb和s2／m26种组合，各亚型所占的比例分别为88．0％（168／191）、7．3％（14／191）、3．1％（6／191）、0．5％（1／191）、0．5％（1／191）和0．5％（1／191）。vacA各亚型在不同H.pylori相关性胃十二指肠疾病中的检出率无显著差异。结论：广东地区H.pylori的vacA基因亚型绝大多数为sla／m2型。不能单纯以vacA亚型作为预测H．pylori感染后临床结局的指标。

Background/Aims: To investigate the epidemiology of vacA gene subtypes of Helicobacter pylori (H. pylori) in Guangdong area and assess the relationships between vacA subtypes and H. Pylori associated gastrointestinal diseases. Methods: 191 H. pylori strains were isolated from patients with different gastric and duodenal diseases in Guangdong area. Bacterial DNA from all the strains were extracted, and the vacA alleles were typed using polymerase chain reaction (PCR) technique with specific primers. Results: Six vacA mosaicisms were found in 191 H. pylori strains, 168 were sla/m2 (88.0%),14 sla/mlb (7.3%), 6 slb/m2 (3.1%), and 1 each was sla/mlb-m2 (0.5%), slb/mlb(0.5%) and s2/m2 (0.5%), respective1y. There were no significant differences in vacA subtypes among various H. Pylori associated gastrointestinal diseases. Conclusions: H. pylori vacA sla/m2 subtype is predominated in Guangdong area. vacA subtypes couldn't be simply used as predictors for disease outcomes.