水稻LOC_Os05g02754基因的分子表征

Molecular Characterization of LOC_Os05g02754 Gene in Rice(Oryza Sativa L.)

已有研究表明,LOC_Os05g02754基因很可能是控制水稻光叶性状的候选基因,光叶性状可能是该基因在第2外显子的5'端非翻译区的一个A→T的单核苷酸变异造成。本研究利用Western blot技术证实LOC_Os05g02754能够翻译成蛋白,但发现光叶和毛叶水稻品种间蛋白表达量不存在显著差异。通过构建该基因与黄色荧光蛋白基因的融合基因载体,并在水稻原生质体中的瞬时表达,将该蛋白定位在叶绿体中;同时,根据预测的二级结构、跨膜特性和信号肽等信息,推测该蛋白为一叶绿体跨膜蛋白。本研究首次试验验证了LOC_Os05g02754基因可以翻译成蛋白,并定位于叶绿体中,但研究结果不支持该基因控制水稻光叶特性。

Previous studies suggested that the LOC_Os05g02754 gene is the candidate gene controlling trichome development and the A→T nucleotide mutation in the 5′ untranslated region of the 2nd exon of this gene might be responsible for the glabrous trait in rice.Western blot analysis demonstrated that LOC_Os05g02754 is translated into protein,however its expression levels in rice leaves were not significantly different between glabrous and pubescent rice cultivars.LOC_Os05g02754 was fused with yellow fluorescence protein gene and the heterologous gene was transiently expressed in rice protoplasts,the results demonstrated that LOC_Os05g02754 is localized in chloroplasts.Bioinformatics analysis of its predicted secondary structure,transmembrane helices and signal peptide also indicated that LOC_Os05g02754 is a protein spanning chloroplast membrane.The present study experimentally demonstrated that LOC_Os05g02754 gene is translated and its protein is localized in chloroplast.However,our results do not support the hypothesis that LOC_Os05g02754 gene controls the pubescent /glabrous trait in rice.