花生种子在不同发育时期的显微结构分析

Microstructure of Peanut Seeds at Different Developmental Stages

花生种子在发育过程中,子叶中贮藏细胞大量合成和积累,了解高、低油花生品种子叶贮藏细胞的特点及差异可为高油品种的定向选择奠定基础。本文采用石蜡切片和徒手切片的方法,运用光学显微镜和激光共聚焦扫描电镜观察了高油品种花U606和低油品种花U17在不同发育时期子叶贮藏物质的积累过程。结果表明,花U606和花U17在花后30d左右出现油体和蛋白体,然后体积也逐渐增大,到最后油体几乎铺满整个细胞。花U606子叶细胞中油体发育的速度明显快于花U17,细胞排列更加致密,其油体数目和截面积也多于花U17。

During the development of peanut,the seed storage cells in cotyledon were synthesized and accumulated.Understanding the characteristics and differences of cotyledon storage cells in high and low-oil peanut can lay the foundation for directional selection of high-oil variety.High-oil Hua U606 and low-oil Hua U17 were selected to observe the accumulation process of the cotyledon storage cells at different developing stages,using optical microscopy and laser scanning confocal microscope.The results showed that oil and protein bodies were found in hua U606 and hua U17about 30 days after flowering,then the volume gradually increased,to the end oil body almost covered the entire cell.Oil body in cotyledon cells of Hua U606 grew faster than Hua U17,arranging more compactly.The number of oil body and cross sectional area of Hua U606 was also higher than Hua U17.