垂枝香柏挥发油的化学成分与抑菌活性分析

Analyses of components and antimicrobial activities of volatile oil from Sabina pingii

【目的】探究垂枝香柏(Sabina pingii)挥发油的成分和药理作用,为其合理推广、开发与应用奠定基础。【方法】采用水蒸气蒸馏法提取垂枝香柏挥发油,并用GC-MS对垂枝香柏挥发油的化学成分进行分析;采用滤纸片扩散和平板涂布法测定垂枝香柏挥发油对金黄色葡萄球菌、沙门氏菌、蜡状芽孢杆菌、卡拉双球菌等4种细菌和白色念球菌、玉米纹枯病菌等2种真菌生长的抑制性,以抑菌圈直径的大小衡量挥发油对供试菌种的抑菌活性。【结果】从垂枝香柏挥发油中共鉴定出63个成分,鉴定成分占总组分的96.63%,其中平均相对含量大于5%的有桧烯(Sabinene)、α-可巴烯-11-醇(alpha-Copaen-11-ol)、烃类含氧衍生物(Hedycaryol)和4-萜品醇(4-Carvomenthenol)等4种物质;垂枝香柏挥发油对6种供试菌种的抑菌圈直径为11.74～14.32mm,均为中度敏感。【结论】垂枝香柏挥发油富含萜类物质,且具有较强的抑菌活性。

【Objective】 The components and pharmacological effects of volatile oil from Sabina pingii were studied to help reasonable exploitation,application and popularization of S.pingii.【Method】 Volatile oil of S.pingii was extracted by steam distillation before its chemical compounds were analyzed by GC-MS.Then,filter paper diffusion method and plate coating method were used to determine its inhibitory effects against 4 bacteria（Staphylococcus aureus,Salmonella setuba,Bacillus cereus,and Diplococcus catarrhalis） and 2 fungi（Streptococcus albus and Rhizoctomicd soldni）.The diameters of inhibition zones were used to indicate the antimicrobial activities.【Result】 63 components were identified from the volatile oil of S.pingii,accounting for 96.63% of the total components.The components with relative contents of lager than 5% on average included sabinene,alpha-copaen-11-ol,hedycaryol,and 4-carvomenthenol.The diameters of inhibition zones of the volatile oil varied from 11.74 to 14.32 mm,which showed a moderate sensitivity.【Conclusion】 Volatile oil of S.pingii was rich in terpenes substances,and had relatively strong antimicrobial activities.