高效液相色谱法测定复方丹参注射液中丹参素的含量

Determination of Danshensu in Compound Danshen Injection by HPLC

目的 :考察不同厂家复方丹参注射液中丹参素含量。方法 :选择复方丹参注射液中活性成份丹参素作为指标 ,用反相高效液相色谱法 ,以甲醇 - 0 .4%醋酸 (5∶95 )作流动相 ,检测波长为 2 81nm。结果与结论 :样品平均加样回收率为 99.71% ,RSD为 0 .96 % ,测定结果不同厂家产品差异很大 ,为保证临床用药安全有效 ,应对其有效成份建立控制标准。

Aim To determine the contents of danshensu in compound danshen injectn of different factories.Methods A HPLC method has been developed to determine danshensu in compound danshen injection,with a detection wavelength of 281nm.The analysis was carried out by using MeOH∶0.4% HAC(5∶95) as the mobile phase.Conclusion RSD was 0.96%,the recovery was 99.71%.The result was different to different factorys' products.The method of quality control should be estabilished.