HPLC法测定复方感冒分散片中4种有效成分的含量

Determination of Four Effective Constituents in Compound Ganmao Dispersion Tablet by HPLC

目的 :测定复方感冒分散片中 4种组分的含量。方法 :采用高效液相色谱法 ,色谱柱为氰基柱 ,(4.9mm× 2 0 0mm ,粒径10 μm) ,流动相为甲醇∶乙腈∶水∶H3PO4∶三乙醇胺 (48∶2 6∶2 6∶0 .2 5∶0 .0 5 ) ,检测波长 :对乙酰氨基酚为 2 45nm ,伪麻黄碱、右美沙芬、氯苯那敏为 2 10nm。结果 :对乙酰氨基酚、伪麻黄碱、右美沙芬、氯苯那敏分别在 3.2 5～ 32 .5 μg、0 .3～ 3.0 μg、0 .15～ 1.5 μg和0 .0 2～ 0 .2 μg范围内线性关系良好 ,回收率、RSD分别为 99.96 % ,0 .99% ;99.2 6 %、1.0 8% ;99.39%、1.0 2 %和 99.17%、1.10 %。结论 :本方法简便、准确、结果可靠 ,可作为复方感冒分散片的定量分析方法。

Aim To determine the concentrations of paracetamol,pseudoephedrine hydrochloride,dextromethorphan hydrobramide and chlorphenamine maleate in compound gammao dispersion tablet.Methods A HPLC method was used. Chromatographic conditions included Hypersil CN column(4.9mm×200mm,φ10μm) and the mobile phase consisting of methaol -acetonitrile-water-H 3PO 4-triethylamine (48∶26∶26∶0.25∶0.05).The detection was set at 245nm for paracetamol and 210nm for pseudoephedrine hydrochloride,Dextromethorphan Hydrobramide and chlorphenamine maleate. Results The calibration curves were linear in the range of 3.25～32.5μg for paracetamol,0.3～3.0μg for psendoephedrine hydrochloride, 0.15～1.5μg for Dextromethorphan Hydrobramide,0.02～0.2μg for chlorphenamine maleate .The average recoveries of paracetamol,pseudoephedrine hydrochloride,Dextromethorphan Hydrobramide and chlorphenamine maleate were 99.96%(RSD=0.99%),99.26%(RSD=1.08%),99.39%(RSD=1.02%)and 99.17% (RSD=1.10%),respectively.Conclusion This method was found to be simple, quick and accurate for the determination of the four constituents in compound ganmao dispersion tablet.