自发性高血压大鼠中医证候及其理化指标相关性研究

On TCM Syndrome and Biological Mechanism of Spontaneously Hypertensive Rats

目的:探寻自发性高血压大鼠(SHR)稳定的证候时间窗及与理化指标的相关性,为深入研究高血压证候生物学机制奠定基础。方法:动态采集SHR和Wistar大鼠宏观表征、血压及行为学试验,Elisa和免疫组化法测定血清NE、NO及脑内NE含量。基于临床证候诊断标准进行动物表征等效转化判别证候属性。结果:SHR14～18周龄表现为肝火亢盛证,与10～12周龄SHR及同周龄wistar大鼠相比,血清NE升高、NO减少和脑内NE升高(P<0.01)。结论:SHR稳定的肝火亢盛证为14～18周龄,与血清NE、NO和脑内NE含量变化密切相关。

Objective：To investigate the stable TCM syndrome window in spontaneously hypertensive rats （SHR） associated with physical and chemical indexes and paves the way for further research on syndrome biological basis of hypertension. Method： We dynamically collected macro characterization, blood pressure, and behavioral tests of SHR and Wistar rats. NE and NO content in serum and brain were detected by Elisa and immunohistochemical method. Syndrome differentiation was conducted by equivalent transformation of animal characterization and clinical symptoms based on the clinical syndrome diagnostic criteria. Result： The 14 to 18 week-old SHR showed stable hyperactivity of liver fire syndrome. Compared to 10-12 week-old SHR and the same week-old wistar rats,14 to 18 week-old SHR serum and brain NE increased, while serum NO reduced（P0.01）. Conclusion： The stable time window of hyperactivity of liver fire syndrome in SHR is 14 to 18 weeks, associated with the increase of serum and brain NE and reduction of serum NO.