竹节参总皂苷对慢性脑缺血大鼠海马GFAP、GAP-43表达的影响

Effects of Total Panax japonicus Saponins on Glial Fibrillary Acidic Protein(GFAP) and Growth Associated Protein-43(GAP-43) in Chronic Cerebral Ischemia Rats

目的:观察竹节参总皂苷(TRPJS)对慢性脑缺血大鼠海马胶质纤维酸性蛋白(GFAP)和神经生长相关蛋白(GAP-43)表达的影响。方法:双侧颈总动脉结扎制备慢性脑缺血大鼠模型,竹节参总皂苷灌胃给药30天后,免疫印迹方法测定海马GFAP和GAP-43的表达。结果:慢性脑缺血大鼠海马GFAP表达增强,GAP-43表达有降低趋势;tRPJS可明显降低GFAP表达和增加GAP-43表达(P<0.01)。结论:tRPJS可降低慢性脑缺血引起大鼠海马星形胶质细胞过度反应,上调生长相关蛋白GAP-43,这可能是其提高学习记忆的作用机制。

Objective：To observe the effect of total rhizoma panacis japonica saponins（tRPJS） on the expression of glial fibrillary acidic protein （GFAP） and growth-associated protein （GAP-43） in hippocampus of chronic cerebral ischemia rats. Methods：The chronic cerebral ischemia model rats were established by ligation of bilateral cephalic artery. Expressions of GFAP and GAP-43 protein in hippocampus region were measured by immune imprinting methods after intragastric administration of tRPJS for 30 days. Results： Expression of GFAP protein in hippocampus region increased while expression of GAP-43 protein in hippocampus region decreased obviously in model group. The tRPJS can decrease the expression of GFAP protein in hippocampus region and can increase the expression of GAP-43 protein in hippocampus region significantly （P0.01）.Conclusion： The tRPJS can inhibit the overreaction of astroglia cell and up-regulate the expression of GAP-43 protein in hippocampus region in chronic cerebral ischemia rats. This may be one of the mechanisms of improving the capability of learning and memory.