HPLC-ELSD法同时测定复方丹参片中4种皂苷的含量

Simultaneous determination of four saponins in Fufang Danshen Tablets by HPLC-ELSD

目的:建立复方丹参片中三七皂苷R_1、人参皂苷Rg_1、人参皂苷Rb_1、人参皂苷Rd的含量测定方法。方法:采用HPLC-ELSD法,使用C_18色谱柱,以乙腈-水为流动相进行梯度洗脱,流速1.0mL·min^-1,柱温25℃,蒸发光散射检测器漂移管温度50℃,载气流速1.5L·min^-1。结果:目标峰分离良好,三七皂苷R1、人参皂苷Rg_1、人参皂苷Rb_1、人参皂苷Rd的线性范围分别为5.14～102.80μg·mL^-1、20.16～322.56μg·mL^-1、40.24～402.40μg·mL^-1、10.06～100.60μg·~mL-1,平均回收率分别为98.1%、96.7%、101.5%和98.1%。结论:该方法简便、准确、耐用性好,可用于复方丹参片中三七皂苷R_1、人参皂苷Rg_1、人参皂苷Rb1、人参皂苷Rd含量的测定。

Objective：To establish HPLC-ELSD method to determine the contents of notoginsenoside R1, ginsenoside Rg1, ginsenoside Rb1 and ginsenoside Rd in Fufang Danshen Tablets. Methods：C18 columns were used with gradient elution of acetonitrile-water as mobile phase by flow rate of 1.0mL·min-1. The column temperature was 25℃. The ELSD heater temperature was 50℃. Air flow rate was 1.5L·min-1. Results： The measured peaks were completely separated from the other components in the peak. The linear ranges of notoginsenoside R1, ginsenoside Rg1, ginsenoside Rb1 and ginsenoside Rd were 5.14~102.80μg·mL-1, 20.60~322.56μg·mL-1, 40.24~402.40μg·mL-1 and 10.06~100.60μg·mL-1 respectively. The average recoveries of notoginsenoside R1, ginsenoside Rg1, ginsenoside Rb1 and ginsenoside Rd were 98.1%, 96.7%, 101.5% and 98.1% respectively. Conclusion： The method is simple and accurate with good robustness. It can be used for the determination of notoginsenoside R1, ginsenoside Rg1, ginsenoside Rb1 and ginsenoside Rd in Fufang Danshen Tablets.