摘要
目的探索ｍｄｍ－２扩增与胃癌及其淋巴转移的关系。方法用ＤＣ－ＰＣＲ技术定量分析３２例胃癌及转移灶中ｍｄｍ－２扩增。结果 ｍｄｍ－２在转移淋巴结中的扩增频率（５７． １％）高于胃原发癌中的扩增频率（３７．５％），３例淋巴管内有瘤栓、淋巴结转移阴性的原发癌灶中发现ｍｄｍ－２扩增。结论ｍｄｍ－２扩增与胃癌的淋巴转移关系较密切，它可能成为监测胃癌淋巴转移的潜在性分子标志物。
Objective To study the relationshap between the amplification of mdm-2 and gastric cancer (GC), and its lymph node metastasis. Methods The amplification of mdm-2 in the 32 primary gastric carcinoma and metastatic lymph nodes were detected by the differentially competitive PCR (DC- PCR). Results The frequency of mdm-2 amplification was higher in the metastatic lymph nodes than in the primary carcinoma(57. 1% and 37. 5% respectively). Mdm-2 amplification was founded in 3 specimens of primal foci with lymphatic tube carcmoma thrombus but without lymph node metastasis. Conclusion The amplification of mdm-2 is well correlated with lymph node metastasis of GC. Mdm-2 gene may be served as a potential genetic marker for monitoring lymph name metastasis of GC.
