安徽白山羊不同组织miR-143表达丰度的Real-time PCR检测

Quantitative Analysis of miR-143 in the Tissues of Anhui White Goat through Real-time PCR Strategies

MicroRNA(miRNA)是一类重要的非编码小RNA分子,其通过对靶基因的转录后调控广泛参与真核生物生殖、发育、病毒防御、细胞增殖和凋亡、脂肪代谢等多种生命活动。采取比较基因组学方法,在对其他7种哺乳动物直向同源基因序列分析的基础上,设计特异性引物,应用实时定量PCR技术测定安徽白山羊心、肝、脾、肺、肾、卵巢、子宫、输卵管、肌肉等9种组织中miR-143的表达情况。结果表明,依据同源基因设计的引物,可以进行山羊miR-143基因特异性检测。安徽白山羊miR-143表达丰度以肺脏最高,肝脏最低;7种组织相对表达量由高到低依次为:肺、子宫、心、脾和卵巢、输卵管和肌肉、肾、肝。研究结果为进一步研究miR-143在山羊组织分化及生长发育过程中的作用提供了借鉴。

MicroRNAs （miRNAs）, a group of important noncoding small RNAs, are involved in diverse aspects of eukaryotic biology including reproduction, development, pathogenesis, cell proliferation, apoptosis and lipometabolism by pairing, albeit imperfectly, to mRNAs, which mainly affects target-specific post-transcriptional repression. Taking a comparative genomics approach, we designed a specific primer for q-PCR technology based on the analysis of miR-143 homologous sequences of other 7 species of mammals to detect the expression levels of miR-143 in 9 hircine tissues such as heart, liver, spleen, lung, kidney, ovary, uterus, oviduct and muscle. The results showed that the primer designed basing on homologous sequence could be used for miR-143 gene-specific detection;the highest expression level of miR-143 was detected in lung and the lowest was in liver , the descending order of expression levels of miR-143 was as follows： lung, uterus, heart, spleen and ovary, oviduct and muscle, kidney, liver. This study could provide a reliable reference to further studies of roles of miR-143 in goat development and histodifferentiation.