克隆清原马鹿的微卫星鉴定

Microsatellite DNA Analysis of Cloned Qingyuan Deer

为对体细胞克隆马鹿进行遗传鉴定,选取8对在牛中具有多态性的微卫星位点,通过筛选出在清原马鹿中具有高度多态性的引物,分别对体细胞克隆清原马鹿、清原马鹿供体细胞、受体清原马鹿进行微卫星分析。结果表明:清原马鹿的HUJ1177、BM888、BM757、IDVGA37、oarFCB304、RM12等6个微卫星位点的PCR扩增产物经聚丙烯酰胺凝胶电泳后呈现多态性。经PAGE电泳分析,克隆清原马鹿与供体细胞的微卫星DNA基因型完全相同,而与受体清原马鹿的微卫星基因型明显不同。因此,体细胞克隆清原马鹿基因组来源于供体清原马鹿细胞而与受体无亲缘关系;HUJ1177、BM888、BM757、IDVGA37、oarFCB304、RM12等6个微卫星位点可用于克隆清原马鹿的鉴定。

To identify the cloned Qingyuan deer,8 microsatellite loci from Qingyuan deer were selected recipient Qingyuan deer.The results suggested that RM12 were remarkably polymorphic after running on pairs of cow polymorphic microsatellite loci and highly polymorphic and used for the analysis of cloned Qingyuan deer,donor Qingyuan deer, the PCR products of HUJ1177,BM888,BM757,IDVGA37,oarFCB304, the SDS-PAGE . The microsatellite DNA genotype of the somatic cloned deer are the same as the donor,but are different from the recipient deer. These results indicated that the six pairs of microsatallite loci used for analyzing genomes of the cloned deer and the somatic cloned deer came from the donor cell.