GRP78在子痫前期患者胎盘和蜕膜中的表达及意义

Expression of GRP78 in placenta and decidua of the patients with preeclampsia.

目的研究子痫前期患者胎盘和蜕膜中GRP78蛋白的表达及意义。方法采用免疫组化SP法检测子痫前期和正常妊娠孕妇的胎盘和蜕膜中GRP78蛋白的表达,应用IPP6.0对GRP78的表达进行半定量分析。结果 GRP78表达于各组胎盘滋养细胞和蜕膜细胞胞浆中;胎盘中GRP78的表达水平轻度组为0.315±0.068,重度组为0.335±0.079,对照组为0.251±0.043,子痫前期各组与对照组相比P<0.05,差异有统计学意义,轻度组与重度组相比P>0.05,差异无统计学意义;蜕膜中GRP78的表达水平轻度组为0.277±0.032,重度组为0.316±0.059,对照组为0.241±0.053,各组相比P<0.05,差异有统计学意义。结论子痫前期患者胎盘和蜕膜中GRP78的表达水平上调,可能在子痫前期的发生过程中发挥重要作用。

Obiective：To explore expression and significance of GRP78 in placenta and decidua of patients with Preeclampsia.Methods： The placenta and decidua were collected from 15 mild preeclampsia patients,25 severe preeclampsia patients and 25 normal pregnancy women.The expression of grp78 in placenta and decidua were detected by using immunohistochemistry methods.The Image Pro-Plus 6.0 was employed to analyze the expression of grp78.Results： GRP78 were positively expressed in trophoblast and decidua cells.①In placenta,the expression of GRP78 in different PE groups were significantly higher than that in control group（P0.05）.However,There were no significant differences among different PE groups（P0.05）.②In decidua,the expression of GRP78 in different PE groups were significantly higher than that in control group（P0.05）.there were significant differences among different PE groups（P0.05）.Conclusions： The expression of GRP78 increase in the placenta and decidua of preeclamptic patients,and may play an important role in the pathogenesis of preeclampsia.