水溶性维生素的毛细管电泳测定

Simultaneous Determination of Water-Soluble Vitamins with Capillary Electrophoresis

建立了高效毛细管电泳同时测定水溶性维生素制剂中的维生素 B1、维生素 B2、维生素 B6、维生素 B12、维生素 C、烟酸、烟酰胺等的方法。缓冲液为 0 .0 2 mol/ L的硼酸溶液 ,毛细管为 5 0μm× 5 0 cm,检测波长为 190 nm (适用于维生素 B1、维生素 B6、维生素 B12、烟酸、烟酰胺 )和 2 6 5 nm(适用于维生素 B2、维生素 C) ,分离度高 ,方法简便。

A simple, rapid and efficient method for the simultaneous assay of vitamin B1, vitamin B2, vitamin B6, vitamin B12, vitamin C, nicotinic acid and nicotinamide by capillary electrophoresis has been established. The experimental conditions were as follows: the electrophoretic medium was composed of 0.02 mol/L boric acid solution, uncoated capillary used was 50 cm×50 μm id. and detection was carried out at 190 nm (to vitamin B1, vitamin B6, vitamin B12, nicotinic acid and nicotinamide) and 265 nm (to vitamin B2, vitamin C).