实验性病毒性心肌炎组织中连接蛋白43和结蛋白的表达

The expression of connexin 43 and desmin in viral myocarditis

目的 了解病毒性心肌炎时心肌细胞连接蛋白 43和结蛋白表达情况 ,以探讨病毒性心肌炎时心律失常的机制。方法 应用免疫组织化学SABC法 ,对实验性病毒性心肌炎小鼠心肌细胞连接蛋白 43和结蛋白的表达进行了观察。结果 连接蛋白 43和结蛋白在正常小鼠心肌闰盘中定位分布 ,均匀存在 ,后者还在肌节横纹中显示阳性 ;病毒性心肌炎时两者的表达明显减弱甚至阴性。结论 病毒性心肌炎时受累的心肌细胞连接蛋白 43和结蛋白的表达受到抑制 ,可能是导致心肌细胞缝隙连接通讯和收缩功能障碍以致心律失常的一个原因。

Objective To study the cytobiological basis of constriction dysfunction and arrhythmia in cardiac muscle cells with viral myocarditis. Methods The expression of connexin 43 and desmin in cardiac muscle cells of mice with experimental viral myocarditis was determined by immunohistochemistry. Results In normal mice, connexin 43 and desmin are located in the intercalated disks of cardiac muscle, and the latter also revealed a positive immunoreactivity in the cross striations of sarcomeres. In viral myocarditis, the expression of both became much weakened, can even become negative. Conclusion In myocarditis, expression of connexin 43 and desmin in the involved cardiac muscle cells was inhibited, resulting in dysfunction of gap junctional communication and arrhythmia.