FHIT基因与MDR1基因mRNA在鼻咽癌组织中表达的临床意义及关系

The relationship between the FHIT and MDR1 genes in the nasopharyngeal carcinoma tissue

目的检测鼻咽癌组织中FHIT和MDR1 mRNA表达量,并探讨它们与临床病理学之间的关系。方法用实时荧光(real-time)相对定量PCR,检测炎性对照组45例和癌症实验组61例鼻咽部组织。结果 FHIT基因与MDR1基因mRNA的表达量炎性对照组和癌症实验组差异均有统计学意义(P值分别为0.049和0.014)。癌症实验组FHIT基因mRNA表达量低于炎性对照组,并且在癌症实验组中组织学分型、临床分期、局部侵袭中表达差异有统计学意义,其表达量随着癌症进一步发展而降低。癌症实验组MDR1基因mRNA表达量高于炎性对照组(P<0.05),组织学分层中差异有统计学意义(P=0.05),在其他临床病理指标分层中差异无统计学意义(P>0.05),两个基因的相关性分析显示两者之间相关差异无统计学意义(P=0.14)。结论 FHIT基因mRNA表达量在鼻咽癌组织中较低,并随着病情恶化表达降低。MDR1 mRNA在鼻咽癌实验组织中表达较高。FHIT与MDR1在癌症实验组中mRNA表达相关性比较差异无统计学意义。

Objective To explore the relationship between nasopharyngeal carcinoma and mRNA of FHIT and MDR1 gene.Methods Real-time PCR was used to test the two gene′s mRNA expression in 45 inflammatory control group and 61 nasopharyngeal carcinoma group.Results The two gene′s mRNA expression was different between 45 inflammatory control group and 61 nasopharyngeal carcinoma group（P=0.049 and P=0.014 respectively）.mRNA expression of FHIT was related to histological type,clinical stages and invasion stages.It was lower in the nasopharyngeal carcinoma than that inflammatory control group.It declined with the cancer deteriorating.mRNA expression of MDR1 is higher in the 61 nasopharyngeal carcinoma group.There were no correlation between the expression of FHIT mRNA and MDR1 mRNA in nasopharyngeal carcinoma tissue（P=0.14）.Conclusion The expression of FHIT mRNA is lower and MDR1 mRNA is higher in nasopharyngeal carcinoma tissue.There is no correlation between FHIT and MDR1.