免疫功能受损宿主外周血单个核细胞端粒酶活性研究

Telomerase activity of peripheral blood mononuclear cells in patients with acquired immune dysfunction

目的 探讨免疫功能受损宿主外周血单个核细胞 (PBMC)端粒酶活性的表达情况。方法 采用PCR ELISA法测定了 17例健康者、14例慢性乙型肝炎、8例糖尿病和 8例慢性肾功能衰竭患者的PBMC经植物血凝素 (PHA)刺激前后端粒酶的活性水平 ,及重组人白细胞介素 12 (rhIL 12 )对端粒酶活性的影响。结果 (1) 4组静息PBMC有低水平的端粒酶活性表达 (A值分别为 0 5 2± 0 2 8、0 32± 0 2 7、0 41± 0 2 0及 0 43± 0 30 ) ,经PHA刺激后活性明显升高 (1 39± 0 0 9、1 2 1± 0 38、1 38±0 11及 1 36± 0 0 8) ;(2 )慢性乙型肝炎患者静息期PBMC端粒酶活性显著低于健康者 ,但PHA刺激后两者活性差异无显著性 ;(3)未能发现rhIL 12对端粒酶活性的影响。结论 PBMC能表达端粒酶活性 ,慢性乙型肝炎患者的免疫功能缺损可能与其PBMC端粒酶活性下降有关。

Objective To investigate telomerase activity of peripheral blood mononuclear cells(PBMC) in patients with acquired immune dysfunction. Methods Telomerase activity of peripheral PBMC isolated from 4 groups including healthy subjects(17), chronic hepatitis B patients(14), diabetic patients(8) and chronic renal failure patients(8) was assayed with PCR ELISA. Results (1) Telomerase activity was detectable at low levels in resting PBMC of all the 4 groups. It increased obviously after in vitro phytohemagglutinin (PHA) stimulation;(2) Telomerase activity of the resting PBMC in chronic hepatitis B patients was significantly lower than that in normal subjects, and was upregulated to the same level in the both groups after PHA stimulation;(3) No effect of recombinant human interleukin 12 on telomerase activity of activated T lymphocytes was found. Conclusions PBMC may express telomerase activity and the decreased telomerase activity of PBMC in patients with chronic hepatitis B may partially be responsible for immune dysfunction in these patients.