大鼠脑缺血时血中脑细胞胞浆酶的变化

CHANGES OF SERUM CYTOSOLIC ENZYME IN RAT WITH CERBRAL ISCHEMIA

目的 研究大鼠脑缺血时脑细胞胞浆酶含量变化的影响。方法 采用全自动生化分析仪检测血清肌酸激酶 (CK)、肌酸激酶脑型同工酶 (CK-BB)、乳酸脱氢酶 (L DH)以及天门冬氨酸氨基转换酶 (AST)的含量。结果 大鼠脑缺血 2 0 min,血中 CK、CK-BB和 L DH的含量明显高于假手术组 (P<0 .0 5或 P<0 .0 1) ;脑缺血 6 0 min和 12 0 min,血中 CK、CK-BB、LDH和 AST的含量均明显高于假手术组 (P<0 .0 1)。结论 脑缺血时血中胞浆酶的活性增加 。

Objective To study the changes of serum cytosolic enzyme in rat with cerebral ischemia.Methods Autobiochemical analyzer was used to measure the patients' creatine kinase (CK) of serum cytosolic enzyme, creatine kinase of brain band isoenzyme (CKBB), lactate dehydrogenase (LDH) and aspartate aminotransferase (AST).Results In the 20min ischemia group, the activities of serum CK, CKBB and LDH were significantly higher than in the shamoperated group (P<0.05 or 0.01), and in 60min and 120min ischemia groups the activities of CK, CKBB, LDH and AST were all greatly increased in contrast with the shamoperated group (P<0.01).Conclusion The activities of cytosolic enzyme are significantly increased during cerebral ischemia and can be used to detect the severity of the injured cell membrane.