转录因子激活蛋白4、血管内皮生长因子C在甲状腺乳头癌中的表达及意义

Expression and clinical values of AP-4 and VEGF-C in papillary carcinoma of thyroid

目的观察转录因子激活蛋白4(AP-4)、血管内皮生长因子C(VEGF-C)在甲状腺乳头状癌(PTC)中的表达,探讨其在判断PTC淋巴结转移上的临床意义。方法应用免疫组织化学(SP法)技术,检测55例PTC及相应癌旁组织和10例正常甲状腺组织中AP-4、VEGF-C蛋白的表达情况,结合PTC临床病理因素对两种蛋白表达进行分析,以探讨临床意义。结果 AP-4和VEGF-C在PTC癌组织中的表达明显高于癌旁组织及正常甲状腺组织(P<0.05);两种蛋白的表达均与患者年龄、肿瘤包膜完整性、肿瘤临床分期及淋巴结转移有关(P<0.05),与性别及肿瘤直径无明显关系。AP-4和VEGF-C蛋白同时高表达判断淋巴结转移的敏感度为91.7%,特异度为84.2%。结论 VEGF-C、AP-4均可能与甲状腺乳头状癌发生发展有关;且AP-4、VEGF-C高表达可能提示甲状腺乳头状癌侵袭性生物学行为强度,同时检测这两种蛋白的表达在判断甲状腺乳头状癌是否淋巴结转移上有更强的预测作用。

Objective To detect the expression and the clinical values of AP-4 and VEGF-C in papillary carcinoma of thyroid（PTC）. Methods The expressions of AP-4 and VEGF-C were detected in 55 PTC tissues, paraneoplastic tissues, and 10 normal thyroid tissues by immunohistochemical technique. The protein expression were compared with clinical parameter of the PTC tissues, for studying the correlation among the expression of AP-4 and VEGF-C and clinical significance. Results The positive expression rate of AP-4 and VEGF-C in PTC tissues were significantly higher than paraneoplastic tissue or normal thyroid tissues（P 〈 0.05）. The expressions of AP-4 and VEGF-C in the PTC tissues which presented an intimate relationship with age, memebrane integrality, clinical stage and lymph node metastasis（P〈 0.05）, but all of them did not show any correlation to tumor size or patient gender. The expressions of AP-4 and VEGF-C in the PTC tissues showed diagnositic values for lymph node metastasis, the sensitivy was 91.7%, and the specificity was 84.2%. Conclusions The expression of AP-4 and VEGF-C may play important roles in the cervical lymph node metastasis of PTC. AP-4 and VEGF-C detecting together might be useful prognosis marker of cervical lympy node metastasis.