自发性高血压鼠脑底动脉血管活性肠多肽能神经纤维的起源

Origin of the Vasoactive Intestinal Polypeptidergic Nerve on Cerebral Vessels in Spontaneously Hypertensive Rats

应用免疫组织化学 ABC法和神经节切除术 ,观察了 2 0只自发性高血压鼠 (分手术 、 、 组和对照组 )脑底动脉血管活性肠多肽能神经纤维的起源。结果显示 ,对照组脑底血管的大脑前动脉、大脑中动脉、大脑后动脉和基底动脉壁上均可见棕褐争的免疫反应阳性纤维 ,纤维似细曲线状 ,呈网状走行。手术 组作一侧蝶腭神经节切除术 ,双侧脑底动脉主要分支的阳性纤维明显减少 ;手术 组作一侧耳神经节切除术 ,双侧大脑后动脉和基底动脉壁上的阳性纤维密度减少 ;手术 组作一侧颈上神经节切除术 ,基底动脉的阳性纤维减少。结果表明 :自发性高血压鼠一侧脑底动脉主要分支的血管活性肠多肽能神经纤维起源于双侧蝶腭神经节、耳神经节和颈上神经节。

Origin of the vasoactive intestinal polypeptidergic nerves on cerebral vessels in 20 spontaneously hypertensive rats (SHR) (divided into operating Ⅰ、Ⅱ、Ⅲ groups and control group) were studied with ganglionectomy and ABC immunoperoxidase technique, employing vasoactive intestinal polypeptide (VIP) as primary antibody. The immunoreactive positive fibers with the brown lineal structures were observed on the anterior cerebral artery (ACA)、 middle cerebral artery (MCA)、 posterior cerebral artery (PCA) and basilar artery (BA) in control groups. Density on bilateral ACA、MCA、PCA and BA obvious decreased. In operating Ⅱ group with unilateral otic ganglionectomy, the density of the positive fibers on bilateral PCA and BA decreased. In operating Ⅲ group with unilateral superior cervical ganglionectomy, the positive fiber's density on BA decreased. It is suggested that vasoactive intestinal polypeptidergic nerve fibers on unilateral cerebral vessels of the SHR originate from bilateral sphenopalatine, otic, superior cervical ganglion, and the vasoactive intesinal polypeptidergic nerves play an important role in the maintenance and development of the hypertension in SHR.