TGF-βⅡR在大肠癌组织中的表达及临床意义

EXPRESSION OF TGF-βⅡR IN COLORECTAL CARCINOMA AND ITS CLINICAL VALUE

1目的 探讨转化生长因子 β 型受体 (TGF- β R)表达与大肠癌生物学行为的关系。 2方法 应用免疫组化技术检测 5 6例大肠癌术后标本及癌旁组织中 TGF- β R表达情况。 3结果 癌旁组织中 TGF- β R表达阳性率为 96 .6 4% ,原发癌组织表达阳性率为 44 .6 4% ,差异有显著性 (χ2 =30 .78,P<0 .0 1) ;无区域淋巴结转移者阳性率为 72 .0 0 % ,有区域淋巴结转移者阳性率为 2 2 .5 8% ,差异有显著性 (χ2 =13.6 8,P<0 .0 1)。 TGF- β R表达水平与肠壁浸润深度、Duke分期呈负相关。TGF- β R阳性者术后 3,5年生存率分别为 89.2 0 % ,78.15 % ,而阴性者分别为 76 .6 0 % ,43.75 % ,两者比较差异具有显著性 (χ2 =35 .46 ,P<0 .0 1)。 4结论 检测癌组织中 TGF- β R表达状况 ,有助于判断大肠癌生物学行为及预后。

Objective To explore the relationship between transforming growth factor β receptor type Ⅱ gene(TGF β ⅡR) expression and the biological behavior in colorectal carcinoma.\ Methods\ The expression of TGF β ⅡR was examined immunohistochemically in colorectal carcinoma and tissue adjacent to cancer by using polyclonal antibody.\ Results\ The tissue adjacent to cancer was 96.64% positive for TGF β ⅡR and colorectal carcinoma was 44.64% ( χ 2=30.78, P < 0.01 ). Cancer without regional nodal metastasis showed significantly higher positive staining rate than those with nodal involvement ( χ 2=13.68, P < 0.01 ). Expression of TGF β ⅡR was inversely correlated with Dukes stages. Survival rates of 3 and 5 years were 89.20% and 78.15% ,respectively, for TGF β ⅡR positive staining group as compared with 76.60% and 43.75% respectively for negative staining groupe ( χ 2=35.46, P <0.01).\ Conclusion\ The expression of TGF β ⅡR might be an useful index for predicting prognosis of human colorectal carcinoma. [