氯沙坦对大鼠主动脉内皮损伤后内膜厚度影响

THE EFFECT OF LOSARTAN ON INTIMAL THICKENING IN RATS AFTER VASCULAR BALLOON INJURY

1目的 观察球囊损伤大鼠主动脉内皮后内膜增厚的过程及氯沙坦对其影响。 2方法 建立大鼠主动脉内皮剥脱模型 ,并将其随机分为对照组、手术组和氯沙坦治疗组 ,分别于术后第 3,7,14和 2 8天取主动脉组织 ,经苏木精 -伊红染色及图像分析仪观察内膜增厚的情况及氯沙坦对其影响。 3结果 主动脉内皮剥脱后第 3天已有血管平滑肌细胞 (VSMC)的移行、增殖 ,损伤后第 7天内膜已开始增厚 ,损伤后第 14天 VSMC的增殖更为明显 ,损伤后第 2 8天 VSMC的增殖明显减弱 ,细胞外基质增加 ,内膜继续增厚。使用氯沙坦后 VSMC的移行、增殖及内膜增厚明显减轻。 4结论 氯沙坦能抑制血管内皮损伤后 VSMC的移行、增殖 ,减轻内膜增厚的程度。

Objective To investigate the process of intimal thickening and the effect of losartan, an antagonist of angiotensin Ⅱ AT 1 receptor,on it.\ Methods\ Models of aortic endothelial denudation of rats by balloon catheter were made. The rats were randomly allocated into three groups: group 1( n =24) served as a control, group 2( n =24) were given vascular balloon injury, group 3( n =24) were given losartan(30mg/kg·d -1 ) from 1 day before injury to 28 days after injury. The aortic tissues were taken at day 3,7,14 and 28 . The process of intimal thickening and the effect of losartan on it were investigated histologically.\ Results \ The migration and proliferation of vascular smooth muscle cells(VSMC) existed at day 3 after balloon injury. The intimal thickening began at day 7 after injury. The proliferation of VSMC was more significant at day 14. The proliferation of VSMC decreased obviously at day 28,but extracellular matrix increased and the intimal thickening continued. When given losartan, the proliferation of VSMC and the intimal thickening decreased.\ Conclusion\ Losartan can inhibit the migration and proliferation of vascular smooth muscle cells led by endothelial injury and reduce the extent of intimal thickening. [