摘要
建立了桃儿七中鬼臼毒素含量测定的方法,采用色谱柱:Diamonsil C18(100×4.6 mm,5 um;流动相:甲醇-水(40∶60);流速:1.0 mL/min;紫外检测波长:292 nm.在0.05000μg～10.00μg范围内桃儿七中鬼臼毒素进样量与峰面积的线性关系良好(r=0.9972);平均回收率为98.2%,RSD为1.57%;该方法准确、灵敏、重复性好,可用于桃儿七中鬼臼毒素的含量测定.
A determining method for the content of podophyllotoxin in sinopodophyllum hexandrum was established by using Diamonsil C18（100×4.6 mm,5 um）,the mobile phase consisting of methanol-water（40∶60） with a flow rate of 1.0 mL/min,and a UV detector wavelength of 292 nm.A linear range of 0.05 μg-10.00 μg（r=0.9972） about calibration curve of podophyllotoxin was shown.The average recovery was 98.2%,RSD,1.57%.This HPLC method was proved to be accurate,effective and reproductive for the determination of the content of podophyllotoxin in inopodophyllum hexandrum.
出处
《宁夏师范学院学报》
2012年第6期48-49,53,共3页
Journal of Ningxia Normal University
基金
宁夏科技攻关计划项目(2010168178)
