吡拉西坦含量的HPLC测定

Determination of Piracetam by HPLC

采用反相高效液相色谱法测定吡拉西坦的含量。色谱柱 Shim- pack CL C- ODS,内标为甲氧苄啶 ,流动相为甲醇 -乙腈 -水 (5∶ 2∶ 3) ,流速为 1.0 m l/ min,检测波长 2 30 nm,线性范围 40～ 5 2 0 μg/ ml,相关系数为 0 .9998,回收率10 0 .0 2 % ,相对标准偏差 0 .2 8%。

A HPLC method for the determination of piracetam was described, using a Shim pack CLC ODS (4.6 mm×150 mm, 5 μm) column with a mobile phase of methanol acetonitrile water (5∶2∶3) at a flow rate of 1.0 ml/min and UV detection at 230 nm. Trimethoprim was used as internal standard. The correlation coefficient of the calibration curve was 0.9998 in the range of 40～520 μg/ml.