肺血管内皮细胞在大鼠急性肺损伤发生中的作用

Effect of pulmonary vascular endothelial cells on the development of acute lung injury in rats

目的 :观察肺血管内皮细胞受损在大鼠急性肺损伤发病中的作用及地塞米松的影响。方法 :给Wistar大鼠静脉注射脂多糖 (LPS 5mg/kgBW)复制急性肺损伤模型。采用ELISA、放射免疫、原位杂交等多种方法测定肺组织ICAM - 1mRNA表达、iNOS活性、血中TNF -α、NO-2 /NO3-、ACE含量及肺血管通透性、肺泡灌洗液中细胞数、蛋白含量等的变化。结果 :注射LPS后 ,肺血管ICAM - 1mRNA表达增加 ,从 1h开始至 2 4h达高峰。肺组织匀浆iNOS活性升高、肺血管通透性升高、肺泡灌洗液中中性粒细胞数量增加 ,巨噬细胞数量减少、蛋白含量增加 ,血中TNF -α、NO2 -/NO3-含量升高而ACE含量下降等变化多在注LPS后 2h明显。预先给予地塞米松对上述多种指标的变化有明显缓解作用。结论 :提示LPS通过损伤肺血管内皮细胞导致急性肺损伤 。

AIM: Effect of endothelial cell on the development of acute lung injury and the prevention of dexamethasone in acute lung injury were observed.METHODS:Rats were divided into three groups:1.Control group.2.LPS group:Venous injection with LPS(5mg/kg body weight),execute respectively at 1 h,2 h,6 h and 24 h after LPS injection. 3.dexamethasone group:intraperitoneal injection with dexamethasone ,1 h before LPS injection,execute after 2 hours after LPS injection.RESULTS: Serum NO,TNF-α levels,lung iNOS activity and lung ICAM-1mRNA expression were increased( P <0.05, P <0.01, vs control group),but serum ACE was decreased( P <0.01).Dexamethasone could improve all the changes above mentioned.CONCLUSION:Endothelial cell played a vital role in the development of acute lung injury and dexamethasone could prevent acute lung injury.