电解性自由基对离体豚鼠冠脉内皮与气道上皮的损伤作用

Effects of electrolyzed free radicals on endothelium of isolated guinea pig coronary and epithelium of airway tube

目的和方法 :采用电解Krebs灌流液产生自由基的方法 ,观察电解性自由基对豚鼠离体心脏冠脉内皮与气道上皮功能的影响。结果 :10mA直流电 ,持续 1- 2min电解灌流液产生的自由基导致离体心脏冠脉压升高约 4 4kPa ,同时使心肌收缩力下降 ,心肌组织过氧化脂质含量增高 ,而SOD活性降低 ;使离体支气管肺灌流压升高约 2 2 0kPa ,并对磷酸组胺反应性增高 3倍 ;电解灌流液的作用可被羟自由基清除剂甘露醇、DMSO抑制。结论 :电解性自由基可引起冠脉内皮与气道上皮损伤 。

AIM and METHODS:To study the damage effects of free radicals from electrolyzed krebs solution(direct current,10 mA,1-2 min) on isolated guinea pig coronary and airway tube. RESULTS:In Langendorff's perfused guinea pig hearts,the electrolyzed free radicals increased coronary perfusion pressure(4.4±1.2) kPa,inhibited myocyte contractility [(0.8±0.8) g vs (2 9±0 6) g, P< 0.05],increased TBARS level and decreased SOD activity.In isolated perfused lungs of guinia pig,electrolyzed Krebs solution promoted significantly the airway perfusion pressure [(0.03±0.01) kPa vs (2.20±0.29) kPa, P< 0.01] and histamine reactivity [(0.65±0.37) kPa vs (2.05±0.25) kPa, P< 0.01]. Hydroxyl radicals scavenger DMSO and natural medicine gypenosides prevented the effects of oxygen free radicals. CONCLUSION: These results indicated that the free radicals by electrolyzation could induce damages of coronary endothelium and airway epithelium.