农杆菌介导GNA基因对水稻的遗传转化

Agrobacterium tumefaciens-Mediated Transformation of Rice for GNA Gene

在 pCAMBIA330 0质粒中插入带有RSs 1启动子的GNA基因 ,并利用农杆菌介导的遗传转化将其导入水稻的微不定芽受体 ,得到了再生植株 .PCR ,Southernblot和Westernblot的检测结果初步证明GNA基因已经整合到水稻的基因组中并得到了表达 .

GNA gene promoted by RSs 1 promoter was constructed into the pCAMBIA3300 plasmid vector, then transformed into micro adventitious bud of rice mediated by Agrobacterium tumefaciens . Regenerated plants were obtained from the transformed bud. The preliminary results of PCR, Southern blot and Western blot for the transgenic plants showed that GNA gene was introduced into the rice cells and expressed.