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Cell trapping and patterning using dielectric-structure-assisted negative dieletrophoresis

Cell trapping and patterning using dielectric-structure-assisted negative dieletrophoresis
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摘要 This paper reports a cell trapping and patterning method using dielectric-pattern-assisted negative dielectrophoresis (dnDEP) that can achieve high-density cell arrays and complicated cell patterns.The dnDEP device consists of two planar metal electrodes and a patterned dielectric layer sandwiched in-between.The dielectric patterns generate non-uniform electric fields when an ac voltage is applied on the two electrodes,and the interaction of polarizable cells and the non-uniform electric fields imposes negative DEP forces on the cells,pushing them to the patterns in the dielectric layer.Using this dnDEP device,human lymphoma cells were successfully patterned in high-density microwell arrays and stellate structures.Thanks to the three-dimensional configuration and the small distance between the electrodes,cell trapping and patterning can be implemented with a voltage of 10 V.This dnDEP device has the advantages of simple configuration,low actuating voltage,and the compatibility with high conductivity physiological media for cell culturing.
出处 《Science China(Technological Sciences)》 SCIE EI CAS 2013年第4期1001-1007,共7页 中国科学(技术科学英文版)
基金 supported in part by the National Natural Science Foundation of China (Grant No. 60871006)
关键词 cell patterning DIELECTRIC NEGATIVE dielectrophoresis (DEP) TRAPPING 介质结构 图案化 细胞 捕获 电泳 非均匀电场 金属电极 构图方法
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  • 1朱树存,易红,倪中华.介电泳辅助的药物洗脱支架载药机理[J].机械工程学报,2006,42(10):165-169. 被引量:2
  • 2Manz A,Graber N,Widmer H M.Miniaturized Total Chemical Analysis Systems:A Novel Concept for Chemical Sensing[J].Sensors & Actuators.B,1990,1:244-248.
  • 3Chou Chia-Fu,Zenhausern F.Electrodeless Dielectrophoresis for Micro Total Analysis Systems[J].IEEE Engineering in Medicine and Biology Magazine,2003,22:62-67.
  • 4Pohl H.Dielectrophoresis[M].Cambridge University Press:Cambridge,1978.
  • 5Green N G,Morgan H.Dielectrophoresis of Submicrometer Latex Spheres[J].Journal of Physical Chemistry B,1999,103(1):41-50.
  • 6Pethig R.Dielectrophoresis:Using Inhomogeneous AC Electrical Fields to Separate and Manipulate Cells[J].Critical Reviews in Biotechnology,1996,16(4):331-348.
  • 7Morgan H,Hughes M P,Green N G.Separation of Submicron Bioparticles by Dielectrophoresis[J].Biophysical Journal,1999,77(1):516-525.
  • 8Becker F F,Wang X B,Huang Y,et al.Separation of Human Breast-Cancer Cells from Blood by Differential Dielectric Affinity[C]Proceedings of the National Academy of Sciences of the United States of America,1995,92(3):860-864.
  • 9Cummings E B,Singh A K.Dielectrophoretic Trapping without Embedded Electrodes[C].Proc.SPIE,2000,4177(29):229-240.
  • 10Cummings E B,Singh A K.Dielectrophoresis in Microchips Containing Arrays of Insulating Posts:Theoretical and Experimental Results[J].Analytical Chemistry,2003,75 (18):4724-4731.

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