摘要
利用 DNA重组技术 ,将经定点突变改造的绿色荧光蛋白基因 ( gfp)克隆到植物表达载体p BI-1 2 1中 ,成功地构建了植物重组表达质粒 p BI-GFP.
The plant recombinant expression plasmid pBI GFP has been successfully constructed by cloning the green fluorescent protein gene which had been optimized by site directed mutagenesis into the plant expression vector pBI 121.The molecular biological technique such as single enzyme insertion was also used in this research.
出处
《华中师范大学学报（自然科学版）》
CAS
CSCD
北大核心
2000年第2期213-215,共3页
Journal of Central China Normal University：Natural Sciences
基金
国家教委留学回国人员启动基金
关键词
绿色荧光蛋白基因
植物重组表达质粒
pBI-GFP
green fluorescent protein gene (gfp)
plant recombinant expression plasmid
gene cloning
