黄芩素抗人巨细胞病毒的体外实验研究

Antiviral Effects of Baicalein on Human Cytomegalovirus in Vitro

目的体外研究黄芩素抗人巨细胞病毒(human cytomegalovirus,HCMV)的效果和安全性。方法细胞病变法和噻唑蓝(MTT)法检测各浓度黄芩素对人胚肺成纤维细胞(HEL)存活率及HCMV抑制率的影响,并与更昔洛韦进行比较。数据输入SigmaPlot软件,经Hill方程曲线拟合计算药物的半数毒性浓度(TC50)和半数有效浓度(EC50)。结果更昔洛韦和黄芩素的TC50分别为353.1"g/ml和151.4"g/ml,EC50分别为62.98"g/ml和14.47"g/ml,治疗指数分别为6和10。结论黄芩素具有体外抗HCMV作用,值得进一步研究。

Objective To study the anti-cytomegalovirus effect and security of baicalein. Methods HEL ceil viability and HCMV inhibitory rate of baicalein in different dosage groups were detected with the cytopathic assay and MTT method,and ganciclovir was used as the control drug for comparison. All data input SigmaPlot statistical software for analyzing 50 % toxic concentrations （TC50） and 50% effective concentrations （EC50）. Results The TC50 of ganciclovir and baicalein were 353.1 μg/ml and 151.4 μg/ml;the EC50 were 62.98 μg/ml and 14.47 μg/ml; TI were 6 and 10,respectively. Conclusion Baicalein has the potential advantages for clinical use and worth further study.