肝癌肝动脉化疗栓塞治疗对缺氧诱导因子2α表达的影响及其临床意义

Expression of Hypoxia Inducible Factor 2 alpha and Its Clinical Significance in Patients with Hepatocellular Carcinoma Treated with Transarterial Chemoembolization

目的:探讨采用肝动脉化疗栓塞(transarterial chemoembolization,TACE)治疗的肝癌患者肝癌组织中缺氧诱导因子2α(hypoxia inducible factor 2alpha,HIF-2α)的表达及其临床意义。方法:采用30例TACE治疗后手术切除的肝癌患者(研究组)的肝癌组织标本以及30例直接手术切除的肝癌患者(对照组)的肝癌组织标本,采用实时荧光定量逆转录-聚合酶链反应(RT-PCR)法、蛋白质印迹法(Western blot)、免疫组织化学(IHC)方法检测肝癌组织中HIF-2α的表达水平并分析其临床意义。结果:研究组肝癌组织中HIF-2α的mRNA和蛋白水平显著高于对照组(P<0.05);肝癌组织中HIF-2α蛋白表达水平与肝癌患者的总体生存时间相关(95%可信区间为0.442～0.972,P<0.05),而与无瘤生存时间无关(P>0.05)。结论:TACE治疗可使肝癌组织中HIF-2α表达水平显著上升,其表达水平与恶性表型相关。

Objective：To investigate the expression of hypoxia inducible factor 2 alpha（HIF-2α） and its clinical significance in patients with hepatocellular carcinoma treated with transarterial chemoembolization（TACE）.Methods：HIF-2α mRNA and protein levels were detected by quantitative real-time reverse transcription polymerase chain reaction and Western blot in the tissues of hepatocellular carcinoma from patients underwent TACE and operation（study group,n=30） and patients merely underwent operation（control group,n=30）.Expression of HIF-2α was further studied using immunohistochemistry.Finally,the clinical significance of HIF-2α was analyzed.Results： Expression levels of HIF-2α mRNA and protein were significantly up-regulated in the study group compared with those in the control group（P0.05）.The level of HIF-2α protein was significantly correlated with overall survival（95 % confidence interval： 0.442~0.972,P0.05）,but not correlated with disease-free survival（P0.05） in patients with hepatocellular carcinoma.Conclusions： TACE treatment significantly increases HIF-2α expression level,and the level of HIF-2α is correlated with malignant phenotypes of hepatocellular carcinoma.