浮游植物叶绿素a含量测定方法的比较测定

Comparison of Two Methods for Phytoplankton Chlorophyll-a Concentration Measurement

本文比较研究了浮游植物叶绿素α含量测定的 2种常用方法——国内常用的丙酮萃取分光光度法和国际上较通用的热乙醇萃取分光光度法 .实验结果显示 ,热乙醇法具有操作简便、快捷 ,萃取完全 ,低毒害等优点 .两种方法有显著统计差异及很好的相关性 ,其回归方程为 :Chla乙醇 =1 .2 61 Chla丙酮 -3 .5( R=0 .998)

This study compared two simplified methods for phytoplankton chlorophyll a concentration measurement-the hot ethanol method which use 90% hot ethanol as the chlorophyll a extraction agent and the cool acetone method which use 90% cool acetone as the chlorophyll a extraction agent. The hot ethanol method is used widely in the world now but the cool acetone method is normally used in China. The results show that the hot ethanol method has more advantages than the cool acetone method, which are faster and easier to handle, more complete extraction and lower toxic. There is significant statistical difference between these two methods. By using the formula Chla ethanol =1.261 Chla acetone -3.5, the cool acetone method data can be transfered to the hot ethanol method data.