三种自身抗体联合检测对系统性红斑狼疮的诊断价值

Application of combined detection of three auto-antibodies in diagnosis of systemic lupus erythematosus

目的探讨ANA、ds-DNA、ENA三类自身抗体免疫指标在系统性红斑狼疮(SLE)诊断中的意义。方法 2010年1月～2012年12月来我院检测治疗的136例患者血清,采用间接免疫荧光法测定ANA、ds-DNA,免疫印迹法测定ENA。结果 136例SLE患者中各种自身免疫抗体的检出率差异较显著,其中以ANA的阳性检出率最高,其次为ds-DNA,ENA中自身多肽抗体的检出率均不相同,以ds-DNA与抗Sm检出率较相近。结论三种自身抗体联合检测明显优于单个项目的检测,可大大提高检测的敏感性,可提高SLE的检出率。

Objective To investigate the diagnostic value of auto-antibodies including antibody to nuclear antigen （ANA）, ds-deoxyribonucleic acid （ds-DNA） and extractable nuclear antigen （ENA） in systemic lupus erythematosus （SLE）. Methods The serum of 136 patients was detected. ANA as well as ds-DNA were tested by indirect im- munofluorescent method （IIF）, and ENA was tested by western blot. Results The detection rate of female SLE patients was higher than that of male patients. In the serum of patients, the detection rate of ANA, ds-DNA and anti-Sm was 97.8%, 69.1% and 53.7%. And the highest detection rate was ANA. Conclusion Combined detection of ANA, ds- DNA and anti-Sm could improve the sensitivity of SLE.