细胞色素CYP2A6基因多态性与COPD易感性的关系

Association between CYP2A6 polymorphism and susceptibility of chronic obstructive pulmonary disease

目的探讨细胞色素CYP2A6基因多态性与COPD易感性的关系。方法应用聚合酶链反应-限制性片段长度多态性法(PCR-RFLP)检测40例COPD患者及37例健康体检者静脉血细胞色素P450 2A6位点等位基因和基因型。结果 1.COPD患者CYP2A6-163C>A位点基因缺失型(del)和野生型(wt)基因型频率为17.5%、82.5%,健康对照组分别为40.5%、59.5%,两组基因分布频率差异有统计学意义(χ2=5.000,P=0.025),携带CYP2A6wt基因型者较携带CYP2A6del基因型者患COPD风险增加(OR=0.31,95%CI=0.109-0.886,P<0.05)。2.在吸烟者中,携带CYP2A6wt基因型者较携带CYP2A6del基因型者患COPD风险增加(OR=0.24,95%CI=0.064-0.920,P<0.05),在不吸烟者中,携带CYP2A6wt、CYP2A6del基因型者之间患COPD风险无明显差异。结论 1.CYP2A6-163C>A基因多态性可能是COPD发病的危险因素。2.野生型(wt)可能为吸烟者患COPD的一个易感因素。

Objective To investigate the association between CYP2A6 polymorphism and susceptibility of chronic obstructive pulmonary disease（COPD）.Methods 40 COPD patients and 37 healthy people were enrolled in this study,and their venous blood cytochrome P450 2A6 allele and genetic type were detected by PCR-RFLP.Results 1.The genotype frequency of gene deletion type（del） and wild type（wt） on CYP2A6-163C A was 17.5% and 82.5% in COPD patients and 40.5% and 59.5% in the controls respectively.The difference of gene distribution frequency had statistical significance（χ2=5.000,P=0.025）.The COPD patients carrying CYP2A6wt genotype had a higher risk than those carrying CYP2A6del genotype did（OR=0.31,95%CI=0.109-0.886,P0.05）.2.Smokers carrying CYP2A6wt genotype had a higher risk than those carrying CYP2A6del genotype（OR=0.24,95%CI=0.064-0.920,P0.05）.There was no significant difference among nonsmokers.Conclusion 1.The polymorphism of CYP2A6-163CA may be the risk factors causing COPD.2.Wild type（wt） may be a predisposing factor of COPD to smokers.