长沙地区63例女性生殖道尖锐湿疣患者人乳头瘤病毒的检测及基因分型

Detection and Genetic Typing of Human Papilloma Virus in 63 Cases of Female Condyloma Acuminata in Changsha Area

【目的】探讨21种人乳头瘤病毒（Humanpapillomavirusea，HPV）型别在长沙地区女性生殖道尖锐湿疣（Condylomataacuminata，CA）患者中的感染情况及分布特点。【方，法】用引物聚合酶链反应（PCR）及基因序列分析的基因分型方法检测长沙地区女性生殖道CA患者HPV型别，分析其分布特点。【结果】经PCR扩增分析67份CA患者活检标本中，63例HPVDNA阳性（88．89％）；PCR阳性标本中，56例成功分型；单一型别感染率为94．64％，复合型别感染率为5．35％；检出的低危型占62．71％，高危型占37．29％。HPV亚型分布情况为：HPV11，占38．98％，其次是HPV16，占35．59％；HPV6的感染率为23．73％；CA患者主要集中于20～40岁的性活跃期，30～39岁年龄组患者的HPV感染率较低，〈30岁的cA患者中，低危型HPV的感染率显著高于高危型HPV感染率。【结论】本地区CA人群中HPV感染率较高，且以单-亚型感染为主，主要感染型别为HPV11、HPVl6和HPV6；HPV各型别的感染率与年龄有一定的关系，该结果为宫颈癌、CA等HPV相关疾病的防治提供了理论依据。

[Objective]To explore the infection status and distribution characteristics of 21 types of human papilloma viruses（HPV） in female condyloma acuminate（CA） in Changsha area. [Methods] Primer-polymer- ase chain reaction（PCR） and genetic typing method based on sequence analysis were used to detect HPV geno- types in female CA in Changsha city. The distribution characteristics were analyzed. [Results] After PCR am- plification, 63 of 67 biopsy samples of CA patients were positive for HPV-DNA（88. 890%） in which 56 HPV genotypes were detected successfully. The positive rates of single and multiple HPV types were 94.64% and 5.35%, respectively. The detection rate of low-risk HPV and high-risk HPV were 62.71% and 37.29%, re- spectively. The distribution of HPV showed that HPVll occupied 38. 98% and the second was HPV16 （35.59 % ）. The infection rate of HPV6 was 23.73 %. The peak time of CA patients were sexually active stage at 20~40 years old. The infection rate of HPV of patients aged 30-39 years old was lower. Among CA pa- tients under 30 years old, the infection rate of low-risk HPV was significantly higher than that of high-risk HPV. [Conclusion] In Changsha area, HPV infection rate of CA patients is higher, and the main infection is single subtype infection. The most prevalent types of HPV are HPV11, HPV16 and HPV6. The prevalence of HPV types has a certain correlation with the age. The results provide theoretical evidence for the prevention and treatment of HPV related diseases such as cervical cancer and CA.