白介素10对伴放线放线杆菌内毒素诱导兔肺巨噬细胞凋亡的影响

The effect ofAa-LPS and IL-10 on apoptosis of rabbit alveolar macrophages

目的:探讨白介素10(IL-10)对伴放线放线杆菌内毒素(Aa-LPS)体外诱导兔肺巨噬细胞凋亡作用的影响。方法:经兔气管肺泡灌洗获得肺泡巨噬细胞,随机分为空白对照组、Aa-LPS组、Aa-LPS+IL-10组。按实验分组加入Aa-LPS(1μg/mL)、IL-10(0.1μg/mL),24h后裂解细胞,荧光定量PCR法检测促凋亡基因bax、p53和caspase-3的表达。结果:Aa-LPS组bax、caspase-3的表达较空白对照组明显升高(P<0.05),p53的表达与空白对照组比较差异无统计学意义(P>0.05)。Aa-LPS+IL-10组bax、p53、caspase-3的表达较Aa-LPS组降低(P<0.05)。结论:Aa-LPS体外对肺巨噬细胞有促凋亡作用,IL-10可抑制Aa-LPS的促凋亡作用,其机制可能与细胞凋亡的线粒体途径有关。

Objective： To investigate the effect of Aa-LPS and IL-10 on apoptosis of rabbit alveolar macrophages （AM） in vitro. Methods： AM were harvested from the bronchoalveolar lavage fluid. These cells were randomly divided into three groups： control group,Aa-LPS group and Aa-LPS ＋ 1L-10 group. Then these cells were given Aa-LPS （1 μg/mL） ,and IL-10 （0.1 μg/mL）. After 24 hours,the cells were harvested to extract RNA. Finally, real-time RT-PCR was used to detect the expression of gene bax, p53 and caspase-3. Results ：The results showed that the expression of bax and caspase-3 in the Aa-LPS group was significantly higher than that of the control group （P 〈 0.05 ）. The expression of p53 was not statistically significant compared with that of the control group （P 〉 0.05 ）. IL-10 treatment significantly inhibited macrophagocyte apoptosis and suppressed the expression of bax ,p53 and caspase-3 （ P 〈 0.05）. Conclusions：Aa-LPS can promote alveolar macrophages apoptosis in vitro and IL-10 can suppress the effect of Aa-LPS. The mechanism seemed related to the mitochondria-dependent pathway.