胆结石小鼠肝脏核受体基因的表达

目的通过胆固醇结石（GS）小鼠肝脏的核受体基因—肝核受体类似物1（1iver receptor homolog 1,LRH-1）、法尼醇受体（farnesoid X receptor,FXR）、类固醇受体（steroid X receptor,SXR）的表达,探讨与胆固醇结石形成关系.方法 C57BL/6雌性小鼠40只,平均出生后10周,重量28-35g,20只一组分为两组,一组予以正常饮食另一组予以高脂饮食喂养,均8周后处死.病理切片检查胆囊壁细胞组织学改变,并分别采用实时定量RT-PCR法和Western Blot法测定肝脏LRH-I、FXR、SXR的mRNA表达.结果胆固醇结石小鼠胆囊壁细胞呈现炎性改变,LRH-I的mRNA表达均高于对照组（P〈0.01）,FXR、SXR的mRNA表达两组差异无统计学意义.结论啮齿动物肝脏LRH-1基因的表达异常与胆固醇结石形成有关,FXR、SXR基因的表达与胆固醇结石形成可能无关.

Objective To investigate the expressions of nuclear receptor gene-1iver receptor homolog 1（LRH-1）,farnesoid X receptor（FXR）and steroid xenobiotic receptor（SXR）gene in cholesterol gallstone（CGS）mice. Methods Forty C57BL/6 mice,20 with cholesterol gallstone（GS）and 20 controls without gallstones（GSF）were enrolled in this study. mRNA and protein expression of LRH-1、FXR and SXR genes were determined by RT-PCR and Western Blot. Results Gallbladder walls of GS mice were thicker with increased stromal granulocyte infiltration. The expression levels of LRH-1 genes were significantly higher in GS mice than in controls（LRH-1：P〈0.01）.But There were no significant differences in protein and mRNA expressions of FXR and SXR gene between two groups. Conclusion The increased expression of LRH-1 gene may be related to GS disease.