日本栗花粉生活力研究

Primary studies on pollen viability of Castanea crenata

快速测定日本栗花粉的生活力,可保证人工杂交育种的有效开展。为此,笔者采用萌发法测定了日本栗3个栽培品种混合花粉萌发的适宜温度,同时进行不同温度贮藏实验。结果表明:日本栗花粉萌发的适宜温度为32～35℃。不同贮藏方式和贮藏时间对花粉的生活力影响差别大,4℃和-20℃的低温条件更适宜花粉保存。

The research aimed to test the pollen viability to improve the probability of crossing. The pollens of Castanea crenata culli- vats were used as materials.Vilro germination method is used for testing the pollen germination.And the pollen was stored at different conditions.The results indicated that the optimum temperature of Castanea crenata pollen germination is 32 - 35 %. The pollen gemai- nation rate is affected by diferent storage methods and time. 4 ℃ and-20 ℃freezing method to preserve polle is more suitable than room temoeratue.