黄酮化合物对金黄葡萄球菌β-内酰胺酶活性影响

Effect of the flavonoids on the activity of β-lactamase from Staphylococcus aureus

试验旨在筛选黄酮类化合物,研究其对金黄葡萄球菌β-内酰胺酶活性影响。利用试管二倍稀释法测定黄酮类化合物对耐药金黄葡萄球菌MIC值;利用K-B纸片法筛选黄酮类化合物;利用反复冻融法提取β-内酰胺酶,并用酶标仪测定β-内酰胺酶活性。结果表明,芦丁、姜黄素和槲皮素分别作用于耐药金黄葡萄球菌后,其β-内酰胺酶活性分别为2.36,2.78和3.05 U.mg-1,与阴性对照组相比差异极显著(P<0.01)。提示黄酮类化合物能降低金黄葡萄球菌β-内酰胺酶活性,能逆转金黄葡萄球菌耐药性。

Effects of the flavonoids on the activity of Staphylococcus aureus lactamase were studied by screening flavonoids. The minimal inhibitory concentration （MIC） of teicoplanin was determined by tube dilution method, flavonoids were screened by means of K-B paper methods.β-1actamase was prepared by repetitive freeze thaw method, enzyme-labelling measuring instrument was used to measure the activity of Staphylococcus aureusβ-1actamase. Rutin, curcumin and quercetin were acted on Staphylococcus aureus respectively, which showed that the activities of β-1actamase were 2.36, 2.78 and 3.05 U mg. Compared with negative control group, differences were remarkable （P〈0.01）. The results show that the flavonoids could decrease the activity of β-1actamase and reverse the drug resistance of staphylococcus aureus.