丹参注射液对大鼠关节软骨细胞合成胶原和蛋白多糖的影响

The Effect of Salvia miltiorrhiza Injection on phenotype Expression of Collagen and Proteoglycan of Chondrocyte

目的通过对体外培养软骨细胞的实验研究,探寻丹参注射液对软骨细胞合成胶原和蛋白多糖的影响。方法对软骨细胞进行原代及传代培养,通过对培养细胞形态学及甲苯胺蓝染色和免疫细胞化学方法鉴定关节软骨细胞,选择适合传代软骨细胞,应用TNF-α干预后比较丹参注射液干预对体外培养软骨细胞Ⅱ型胶原和蛋白多糖的表达变化。结果 2.5%、5%、10%丹参注射液可以增加TNF-α诱导的关节软骨细胞Ⅱ型胶原及蛋白多糖的表达。结论丹参注射液有促进软骨细胞外基质合成的作用,减少软骨细胞分解代谢,对关节软骨起到保护作用。

Purpose Chondrocytes in vitro experimental study, to explore the impact of Danshen injection on the cartilage cells to synthesize collagen and proteoglycan. Methods On cartilage cells and passage of culture through on morphology of cultured cells and toluidine blue staining and immunocytochemical identification of articular cartilage cells, select the appropriate passage of chondrocytes, TNF-alpha compare danshen injection after intervention on chondrocytes in vitro expression of type II collagen and proteoglycan change. Results 2.5% ,5%, 10% Danshen injection can increase the expression of TNF-a -induced articular chondrocytes collagen type II and proteoglycans. Conclusion Danshen injection to promote extracellular matrix synthesis in chondrocytes, reducing the catabolism of chondrocytes, play a protective effect on articular cartilage