摘要
目的建立同时测量精制冠心片中主药有效成分芍药苷和丹参酮ⅡA含量的方法。方法采用ODS-Cl8色谱柱(4.6 mm×250 mm,5μm);流动相采用乙睛-0.2%磷酸溶液进行梯度洗脱;流速1.0mL/min;检测波长270 nm(检测丹参酮ⅡA),10 min,230 nm(检测芍药苷);柱温:室温。结果芍药苷在12.52-125.2μg·mL-1的范围内峰面积积分值与浓度呈良好的线性关系(r=0.9998),平均回收率为100.1%,RSD=2.4%(n=6);丹参酮ⅡA在2.67-26.7μg·mL-1的范围内峰面积积分值与浓度呈良好的线性关系(r=0.9999),平均回收率为100.2%,RSD=1.8%(n=6)。结论该法准确、简便,重现性好,可用于精制冠心片的质量控制。
Objective To establish a method for content determination of paeoniflorin and tanshinone II A in Jingzhi Guanxin Tablet by HPLC. Methods C18 colum(4.6 mm^250 ram, 5 gm) was used with the mobile phase consisted of acetonitrile-0.2%phosphoric acid gradient elution, with flow rate 1.0 mL/min and the detection wavelength 270 nm(detection for tanshinone Ⅱ A), the detection time 10 min,230nm(detection for paeoniflorin),and the column temperature 25 C. Results The linear range for Paeoniflorin and tanshinone Ⅱ A were 12.52-125.2 tig.mL-i (r=0.999 8) and 2.67-26.7gg'mL-i (r=0.999 9), the average recovery was 100.1% (RSD=2.4%,n=6) for Paeoniflorin and 100.2% (RSD=l.8% ,n=6)for tanshinone II A. Conclusion The method was feasible, accurate and reliable. It could be used for the quality control of Jingzhi Guanxin Tablet.
出处
《现代中药研究与实践》
CAS
2013年第1期60-62,共3页
Research and Practice on Chinese Medicines
关键词
精制冠心片
芍药苷
丹参酮ⅡA
含量测定
Jingzhi Guanxin Tablet
paeoniflorin
tanshinone Ⅱ A
Content determination